Genetic diversity of Porphyromonas gingivalis isolates recovered from single "refractory" periodontitis sites.
نویسندگان
چکیده
Multilocus sequence typing and fimA genotyping were performed on Porphyromonas gingivalis isolates from 15 subjects with "refractory" periodontitis. Several sequence types were detected for most individual pockets. The variation indicated recombination at the recA and pepO genes. The prevalence of fimA genotypes II and IV confirmed their association with periodontitis.
منابع مشابه
Genetic structure of populations of Porphyromonas gingivalis associated with periodontitis and other oral infections.
One hundred isolates of the oral pathogenic bacterium Porphyromonas gingivalis were genetically characterized by determining the electrophoretic mobilities of 16 metabolic enzymes and the presence or absence of catalase activity. A total of 78 distinct electrophoretic types (ETs), representing multilocus genotypes, were identified, and cluster analysis placed them in three major phylogenetic di...
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The introduction of multilocus sequence typing (MLST) in infectious disease research has allowed standardized typing of bacterial clones. Through multiple markers around the genome, it is possible to determine the sequence type (ST) of bacterial isolates to establish the population structure of a species. For the periodontal pathogen, Porphyromonas gingivalis, the MLST scheme has been establish...
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OBJECTIVE The goal of this study was to determine the genetic variability of the fimA gene in Porphyromonas gingivalis isolates from Spanish patients. STUDY DESIGN Pooled subgingival samples were taken, processed and cultured in non-selective blood agar medium. Pure cultures of one to six isolates per patient were obtained and PCR and PCR-RFLP were used for fimbrillin gene (fimA) type determi...
متن کاملPrevalence of Porphyromonas gingivalis and Bacteroides forsythus in chronic Periodontitis by Multiplex PCR
Rezaei F1, Chalabi M1, Moghim SH2, Moghareh Abed A3, Faghri J1 1. Instructor, Department of Microbiology, Faculty of Medicine, Lorestan University of Medical Sciences 2. MSc in Microbiology, Medical Biology Recearch Center, Kermanshah 3. Assistant Professor, Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences 4. Assistant Professor, Department of Periodontolo...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 74 18 شماره
صفحات -
تاریخ انتشار 2008